Follicle Wave and Natural Estrus Synchronization Superovulation in Holstein Cows.

  • Manuel E. Soria Parra Faculty of Agricultural Sciences, University of Cuenca, Yanuncay Campus, Ecuador.
  • Carlos A. Soria Parra Faculty of Agricultural Sciences, University of Cuenca, Yanuncay Campus, Ecuador.
  • Daniel Argudo Garzón Faculty of Agricultural Sciences, University of Cuenca, Yanuncay Campus, Ecuador.
  • Guillermo Serpa García Faculty of Agricultural Sciences, University of Cuenca, Yanuncay Campus, Ecuador.
  • Silvana Méndez Álvarez Faculty of Agricultural Sciences, University of Cuenca, Yanuncay Campus, Ecuador.
  • Carlos Torres Inga Faculty of Agricultural Sciences, University of Cuenca, Yanuncay Campus, Ecuador.
  • Guillermo E. Guevara Viera Faculty of Agricultural Sciences, University of Cuenca, Yanuncay Campus, Ecuador.
Palabras clave: bovine, progesterone, estrus, prostaglandin

Resumen

The effects of two superovulation protocols were compared (synchronization of the follicle wave SFW and natural estrus  NE) in embryos collected from Holstein cows. Twenty cows were chosen as donors, body condition of 2.75  -3.5; between 40 and 60 months, with normal cycles and no breeding problems. The cows were randomly assigned to SFW treatment (n=10), and the NE treatment (n=10). The SFW group was synchronized with intravaginal progester-one plus estradiol  benzoate, on day 0, and increasing doses  of FSH for 4 days, twice a day, from  the 4th on. The im-plant was removed on the 6th
day, and prostaglandin was applied. On the 8th day, insemination was made at 6 am and 6 pm. The NE group received  increasing doses of FSH twice a day, during 4 days, from the 10th day. On the 12th day prostaglandin was administered, and insemination took place on the 14th day, at 6 am and 6 pm.  The em-bryos were recovered from the two groups without surgery, 7 days after the first artificial insemination. The values of embryos for transference were 5.7 + 0.76 and 2.8 + 0.31 (P < 0.05) for SFW and NE, respectively.

Citas

ALLER, J. F.; ABALOS, M. C.; ACUÑA, F. A.; VIRGILI, R.; REQUENA, F. y CANCINO, A. K. (2015). Embryo Yield In Llamas Synchronized With Two Different Intravaginal Progesterone-Releasing Devices and Superovulated With eCG. Spanish Journal of Agricultural Research, 13 (3), 3-8.

AMAYA, A. D. (2010). Efeto del diametro del foliculo ovulatorio,tamaño del cuerpo luteo y perfiles de progesterona sobre la tasa de preñez en la hembra receptora de embriones bovinos, 1-190. Tesis en opción al título de Máster en Salud Animal, Bogotá, Colombia.

BARUSELLI, P. S.; DE SÁ FILHO, M. F.; MARTINS, C. M.; NASSER, L. F.; NOGUEIRA, M. F. G.; BARROS, C. M. y BÓ, G. A. (2006). Superovulation and Embryo Transfer in Bos indicus Cattle. Theriogenology, 65 (1), 77-88. Retrieved on
November 24, 2015, fromhttp://doi.org/10.1016/j.theriogenology.2005.10.006.

BELACUBA, F. (2007). Factores que afectan la superovulación en bovino. Retrieved on November 24, 2015, fromhttp://www.produccion-animal.com.ar/informacion_tecnica/transplante_embrionario/17-superovulacion.pdf

BO, G. A.; ADAMS, G. P.; CACCIA, M.; MARTÍNEZ, M.; PIERSON, R. A. y MAPLETOFT, R. J. (1995). Ovarian Follicular Wave Emergence After Treatment With Progestogen And Estradiol In Cattle. Animal Reproduction Science, 39 (3), 193-204.

BO, G. A.; ADAMS, G. P.; PIERSON, R. A. y MAPLETOFT, R. J. (1996). Effect of Progestogen Plus Estradiol-17β Treatment on Superovulatory Response in Beef Cattle. Theriogenology, 45 (5), 897-910.

BÓ, G. A.; TRÍBULO, A. y MAPLETOFT, R. J. (2011). Nuevos protocolos de superovulación para programas de transferencia de embriones en bovinos. SPERMOVA, 1(1), 26-33.

BÓ, G., y NASSER, L. (2005). Efficiency of Programs that Control Follicular Development and Ovulation for the Donor Superovulation without Estrus Detection. Minneapolis, EE.UU.: Proc Joint Mtg Am Embryo Trans Assoc & Can Embryo Trans Assoc.

CERRI, R.; CHEBEL, R.; RIVERA, F.; NARCISO, C.; OLIVEIRA, R.; THATCHER, W. y SANTOS, J. (2011). Concentration of Progesterone during the Development of the Ovulatory Follicle: I. Ovarian and Embryonic Responses. Journal of Dairy Science, 94 (7), 3342-3351.

FAIR, T. y LONERGAN, P. (2012). The Role of Progesterone in Oocyte Acquisition of Developmental Competence. Reproduction in Domestic Animals, 47 (4), 142-147.

GOULDING, D.; WILLIAMS, D. H.; DUFFY, P.; BOLAND,
M. P. y ROCHE, J. F. (1990). Superovulation in Heifers given FSH Initiated Either at Day 2 or Day 10 of the Estrous Cycle. Theriogenology, 34 (4), 767-778.

JIMÉNEZ, C. (2009). Superovulación : estrategias, factores asociados y predicción de la respuesta superovulatoria en bovinos superovulation: strategies, associated factors, and prediction of the superovulatory reponse in cows. Revista de la Facultad de Medicina Veterinaria y de Zootecnia (Universidad Nacional de Colombia), 56 (3), 195-214. Retrieved on November 24, 2015, from http://www.revistas.unal.edu.co/index.php/remevez/article/view/13769.

LUCA y CASTRILLÓN, M. (2011). Respuesta a la superovulación y calidad de los embriones en bovinos lecheros de elevado mérito genético, uso de diferentes protocolos. engormix. Retrieved on November 24, 2015,
fromhttp://www.engormix.com/MA-ganaderia-carne/genetica/foros/respuesta-superovulacion-calidad-embriones-t22419/103-p0.htm.

MAPLETOFT, R. J.; BENNETT STEWARD, K. y ADAMS,
G. P. (2002). Recent Advances in the Superovulation in Cattle. Reproduction Nutrition Development, 42 (6), 601-611.

RIVERA, F. A.; MENDONCA, L. G. D.; LÓPEZ, G.; SANTOS, J. E. P.; PÉREZ, R. V.; AMSTALDEN, M., et al. (2011). Reduced Progesterone Concentration During Growth of the First Follicular Wave Affects Embryo Quality but Has No Effect on Embryo Survival Post Transfer In Lactating Dairy Cows. Reproduction, 141 (3), 333-342.

SALHAB, M.; TOSCA, L.; CABAU, C.; PAPILLIER, P.; PERREAU, C.; DUPONT, J.; et al. (2011). Kinetics of Gene Expression and Signaling in Bovine Cumulus Cells Throughout IVM in Different Mediums in Relation to Oocyte Developmental Competence, Cumulus Apoptosis and Progesterone Secretion. Theriogenology, 75 (1), 90-104.

STRINGFELLOW, D. A. y SEIDEL, S. M. (2000). Manual
de Transferencia de Embriones (3ra ed.). Sociedad Internacional de Transferencia de Embriones.
Publicado
2017-06-29
Sección
Genética y Reproducción