Enterotoxigenic Escherichia coli Antibio-Resistance Induced by Copper.

  • Guillermo Barreto Argilagos Faculty of Industry Applied Sciences, Ignacio Agramonte Loynaz University of Camagüey, Cuba.
  • Herlinda de la Caridad Rodríguez Torrens Faculty of Agricultural Sciences, Ignacio Agramonte Loynaz University of Camagüey, Cuba.
  • Herlinda de la Caridad Barreto Rodríguez Carlos Juan Finlay Medical Sciences University, Camagüey, Cuba.
Palabras clave: antimicrobials, environment, enteropathogens, growth promoters, swine, tolerance

Resumen

Cupric sulfate is widely used as growth promoter for swine productions, in spite of its ecological risks. The purpose of this paper was to evaluate the in vitro effect of copper as inducer of antibio-resistance in Enterotoxigenic. E. coli. A-1 (O149: K91: K88ac) and E68-I (O141: K85: K88ab) reference strains were grown in Mueller-Hinto agar, according to the concentration gradient technique with weekly increasing values, from 5 to 50 mg/mL of CuSO 4. Both bacteria were cultured for three weeks; the controls were cultured parallely, in the same medium, without the inducer. Then, antibiograms were made to evaluate sensitivity against gentamicin, kanamycin, tetracycline, chloramphenicol, carbenicilin, and nalidixic acid. The results from the strains challenged with salt showed halos compatible to resistant phenotypes, in contrast to the controls (all sensitive). The results evidence the capacity of copper to induce in vitro bacterial antibio-resistance, which must be considered by producers when copper salts are used as growth promoters. In addition to ecological damages, antimicrobials lose effectiveness in human and animal therapeutics.

Citas

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Publicado
2017-05-10
Sección
Salud Animal