Indicators correlated with the strength of the inoculum of bovine faeces in in vitro gas production
Abstract
Using bovine feces as inoculums for gas in vitro production poses a limitation concerning the inoculum power variability. Thus, finding indicators to fastly characterize and control the inoculum power could make it more reliable. This paper is aimed at determining the relationship of washed cells optical density and biomass, and inoculums + mineral medium mixture viscosity with gas in vitro volume. Cells were washed by centrifugation at 1 000 rpm . Viscosity was measured by the time a syringe is emptied. A significant correlation (P < 0,01) was detected between gas in vitro volume produced during 48 h and washed cells optical density and biomass value of 550 nm . No correlation was found for mixture viscosity. Results suggest the possibility of using optical density at different dilution levels to control inoculum power. Optical density is fairly easy to measure.
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References
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