Correlation Between in situ Rumen Degradability and in vitro Gas Production When Using Bovine Feces as Inoculum
Abstract
With the aim of assessing the correlation between in situ rumen degradability and in vitro gas production using bovine feces, dried samples of Leucaena leucocephala, Pennisetum purpureum, and Panicum maximum were incubated during 8; 16; 24; 48; 72, and 96 h within the rumen of two cross-bred adult bulls, fistulated at the dorsal rumen sac, which grazed about 10 hours/per day and remained stabled the rest of the time. As inoculum for gas production, fresh feces with less than 3 hours of being voided, mixed with a buffered mineral medium in a 1 + 3 rate were used. The produced gas was collected in 100 mL gauged syringes. The lag time, potential volume of gas (B), and rate of degradation parameters (C) were estimated. By these volume-time data B value is smaller for P. maximun and C value is greater for L. leucocephala. There is a linear correlation (P<0,01) between in vitro gas production with already voided feces and in situ rumen degradability which suggests the possibility of using the former technique in predicting digestibility without affecting animals condition.
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References
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