Cryofactura to obtain necrotoxins produced by necrotizing E. coli
Abstract
Cryofacture was substituted for mitomycine C to obtain cytolitic necrotic factors (CNF) out of six hemolytic and P fimbria-carrier E. coli strains (two of them isolated from meals and four from diarrheic children under five years old). Differentiation of CNF1 and CNF2 toxins was performed through foot mouse inoculation. The six E. coli strains proved their necrotic character: five producing CNF2 and the other, CNF1. Except CNF2+ strain, the remaining ones were lethal. Results showed the possibility of isolating CNF necrotoxins through cryofrcture. Therefore, this method can be an option to study necrotic E. coli in veterinary medicine, taking into account the role played by bovines as a reservoir of this potentially zoonosic category.
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References
BARRETO, G.; O. JIMENEZ Y S. DIAZ: E. coli verotoxigénico (VTEC): una nueva va- riedad, un nuevo riesgo. Revista de Producción Animal, 10 (anuario): 5-26, 1997-1998.
BARRETO, G.; M. SEDRÉS, A. ORTIZ Y M. RICARDO: Esquema para el diagnóstico de E. coli enterohemorrágico y otras categorías enteropatógenas a partir de alimentos, Revista de Producción Animal, 11 (anuario): 39-43, 1999.
BARRETO, G.; M. SEDRÉS, A. ORTIZ Y M. RICARDO: Categorías enteropatógenas de
E. coli en alimentos, Rev. prod. anim., 12: 87-90, 2000a.
BARRETO, G.; R. I. HERNÁNDEZ, A. ORTIZ, Y Y. SANTIAGO: Esquema para el dia- gnóstico de ECEH y otras categorías enteropatógenas de E. coli a partir de pacientes de EDA, Archivo Médico de Camagüey, 5 (1), 2000b. [Publicación electrónica].
BARRETO, G. Y T. BENÍTEZ: E. coli enterohemorrágico (ECHE). Algunas consecuen- cias de su presentación en el humano, Archivo Médico de Camagüey, 4 (2), 2000, [Pu- blicación Electrónica].
BARRETO, G.; R. I. HERNÁNDEZ, A. ORTIZ Y Y. SANTIAGO: Presencia de E. coli
enteropatógenas en pacientes con diarrea aguda, Archivo Médico de Camagüey, 5(2):
, [Publicación electrónica].
BLANCO, J.; E. A. GONZÁLEZ, S. GARCÍA, M. BLANCO, B. REGUEIRO, E I. BERNÁZDEZ: Production of Toxins by E. coli Strains Isolated from Calves with Diar- rhoea in Galicia (North-Western Spain), Vet. Microbiol., 18: 297-311, 1988.
BLANCO, J. Y M. BLANCO: ETEC, NCEC y VTEC de origen humano y bovino, Pato- génesis, epidemiología y diagnóstico microbiológico, Servicio de Publicaciones Diputa- ción Provincial San marcos, pp. 35-48, 71-77; 104-107; 115-120; 173-176; 207-209,
-239; 306-308; 310-316, Galicia, 1993.
BLANCO, J. E.; M. BLANCO Y J. BLANCO: ETEC, VTEC en alimentos y muestras clíni- cas. Papel de los animales como reservorios de cepas patógenas para el hombre, Mi- crobiología SEM 11:97-110, 1995.
BOQUET, P.: The Cytotoxic Necrotizing Factor 1 (CNF1) from escherichia coli, Toxicon,
(11): 1673-1680, 2001
CAPRIOLI, A.; B. FALBO, L. G. RODA, P. M. RUGGERI Y C. ZONA: Partial Purification and Characterization of an E. coli Toxic Factor that Induces Morphological Cell Altera- tion, Infect. Immun., 39: 1300-1306, 1983.
CAPRIOLI, A.; G. DONELLI, B. FALVO, R. POSSETI, L. G. RODA, G. ROSCETI Y P. M. RUGGERI: A Cell-Division Active Protein from E. coli, Biochem. Biophys. Res. Com- mun, 118: 587-593, 1984.
DE RYCKE, J.; E. A. GONZÁLEZ, J. BLANCO, E. OSWALD, M. BLANCO Y R. BOIVIN: Evidence for Two Types of Cytotoxic Necrotizing Factor in Human and Animal Clinical Isolates of E. Coli, J. Cli. Microbiol., 28: 694-699, 1990.
GONZÁLEZ, E. A. Y J. BLANCO: Serotypes and Antibiotic Resistance of Verotoxigenic (VTEC) and Necrotizing (NTEC) E. Coli Strains Isolated from Calves with Diarrhoea, FEMS Microbiol. Lett., 60: 31-36, 1989.
NATARO, J. P. Y J. B. KAPER: Diarrheagenic Escherichia coli. Clinical Microbiology
Review, 11 (1): 142-201, 1998.
RIPPERE-LAMPE, K. E.; M. LANG, M. CERI, E. OLSON, H. A. LOCKMAN, Y A. D. O'BRIEN: Cytotoxic Necrotizing Factor Type 1-Positive Escherichia coli Causes In- creased Inflammation and Tissue Damage to the Prostate in a Rat Prostatitis Model, Infect. Immun., 10: 6515-6519, 2001.
RUGGERI, P. M.; C. FIORENTINI, A. CAPRIOLI, G. ARANCIA, V. FALBO Y G. DONELLI: Hep-2 Cells Multinucleation Induced by E. Coli Cytotoxic Factors. IRCS Med. Sci., 14: 833-834, 1986.
TEVECHIO, A. T.; L. R. MARQUES, C. M. ABE Y T. A. GOMES: Detection of Necrotiz- ing factor types 1 and 2 among Fecal Escherichia coli Isolated from Brazilia Children with and without Diarrhea, Mem. Inst. Oswaldo Cruz, 99 (1): 81-83, Feb, 2004.
VAN BOST, S.; M.H. BABE, E. JACQUEMIN Y J. MAINIL. Characteristics of Necrotoxi- genic Escherichia coli Isolated from Septicemic and Diarrheic Calves between 1958-
, Vet. Microbiol, 82 (4): 311-320, 2001.
VAN BOST, S.; S. ROELS, E. OSWALD Y J. MAINIL: Putative Role of the CNF2 and CDT111 Toxins in Experimental Infections with Necrotoxigenic Escherichia coli Type 2 (NTEC1) Strains in Calves, Microbes Infect., 5 (13): 1189-1193, 2003.
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