Controlled Breeding of Coccoloba cowellii Britton (Polygonaceae), a Threatened Endemic Species
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Resumen
Context: Coccoloba cowellii Britton is an endemic plant to Camaguey province, Cuba, which has been reported in critical danger of extinction, though phytochemical studies have revealed the presence of promising compounds with antioxidant activity. Hence, the need for standardizing the potential economic exploitation of the species through preservation has encouraged scholars to study the features of the seeds and germination.
Aim: To assess C. cowelli seed dormancy, and evaluate their germination under controlled conditions.
Methods: Anatomical and physiological evaluation of C. cowellii seed dormancy. A standard factorial experiment was designed to evaluate the germination capacity under controlled conditions, consisting of three factors, one with two levels, and the other with three levels. All the possible combinations were evaluated, totaling 18 experimental units.
Results: The presence of non-dehydration sensitive C. cowellii seeds was checked, along with the absence of factors that can delay their physiological maturity. There were differences in terms of germinative efficiency resulting from scarification as pre-germinative treatments, rather than by inhibition. A significant decrease was observed 180 days following the harvest, while the best results were achieved with the utilization of substrate collected from the natural habitat.
Conclusions: C. cowellii seeds can be dehydrated and stored at room temperature to preserve embryo viability of at least 50% of them. The feasibility of germination under controlled conditions favored the implementation of ex-situ controlled steps, the generation of plant material for further research, and the existence of a potential for sustainable production.
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Resumen
Context: Coccoloba cowellii Britton is an endemic plant to Camaguey province, Cuba, which has been reported in critical danger of extinction, though phytochemical studies have revealed the presence of promising compounds with antioxidant activity. Hence, the need for standardizing the potential economic exploitation of the species through preservation has encouraged scholars to study the features of the seeds and germination.
Aim: To assess C. cowelli seed dormancy, and evaluate their germination under controlled conditions.
Methods: Anatomical and physiological evaluation of C. cowellii seed dormancy. A standard factorial experiment was designed to evaluate the germination capacity under controlled conditions, consisting of three factors, one with two levels, and the other with three levels. All the possible combinations were evaluated, totaling 18 experimental units.
Results: The presence of non-dehydration sensitive C. cowellii seeds was checked, along with the absence of factors that can delay their physiological maturity. There were differences in terms of germinative efficiency resulting from scarification as pre-germinative treatments, rather than by inhibition. A significant decrease was observed 180 days following the harvest, while the best results were achieved with the utilization of substrate collected from the natural habitat.
Conclusions: C. cowellii seeds can be dehydrated and stored at room temperature to preserve embryo viability of at least 50% of them. The feasibility of germination under controlled conditions favored the implementation of ex-situ controlled steps, the generation of plant material for further research, and the existence of a potential for sustainable production.
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Alonso, W. (2018). Evaluación de tres tratamientos pregerminativos y tres sustratos para la producción de semilleros de guayacán (Guaiacum sanctum l.), bajo condiciones de invernadero y análisis histológico e histoquímico de su semilla. (Tesis en opción al título académico de Master. Universidad de San Carlos de Guatemala). Guatemala. https://acreditacion.ccqqfar.usac.edu.gt/qf/Anexo11.pdf
Baskin, C., & Baskin, J. (2014). Seeds Ecology, Biogeography, and Evolution of Dorman-cy and Germination. Second Edition. Academic Press is an imprint of Elsevier, San Diego, USA, 12-200. https://books.google.com.cu/books?hl=es&lr=&id=vXfNCgAAQBAJ&oi=fnd&pg=PP1&dq=Baskin,+C.+%26+Baskin,+J.+(2014).+Seeds+Ecology,+Biogeography,+and+Evolution+of+Dormancy+and+Germination.&ots=-ridWgGVye&sig=y7tw1p4ydW_qGN5zs51Xfkgs9kU&redir_esc=y#v=onepage&q&f=false
Castañeda, I. (2014). Taxonomía y filogenia del género Coccoloba (Polygonaceae) en Cuba. (Tesis en opción al grado científico de Doctor en Ciencias Biológicas. Universidad de La Habana), Cuba.
Chávez, M. (2017). Síndromes de domesticación en plantas. Desde el Herbario (CICY), 9, 79–83. http://www.cicy.mx/sitios/desde_herbario/
CITMA (Ministerio de Ciencia Tecnología y Medio Ambiente de la República de Cuba). (2021). Estrategia Ambiental Nacional 2021/2025. https://www.citma.gob.cu/estrategia-ambiental-nacional/
Correia de Araujo, J., Duarte, J., Inajal, M., Ferreira, L., & Marques, V. (2018). Morphometric characterization, germination and conservation of Triplaris brasiliana cham. seeds (Polygonaceae). Ciência Florestal, Santa Maria, 28(3), 949-959. http://dx.doi.org/10.5902/1980509833363
Díaz, M. & Molinelli, M. (2018). Caracterización del fruto y la semilla de Ruprechtia apetala (polygonaceae) en relación con la entrada de agua y la germinación. Bonplandia, 27(1), 5-22. https://www.researchgate.net/publication/326003263
González, L., Palmarola, A., González-Oliva, L., Bécquer, R., Testé, E., & Barrios, D. (Eds.). (2016). Lista roja de la flora de Cuba; Bissea 10 (número especial 1), 1-352. https://www.researchgate.net/publication/309313148_Lista_Roja_de_la_Flora_de_Cuba_-_2016
Lozano-Isla, F., Miranda, P., & Pompelli, M. (2017). Germination behavior of Jatropha curcas L. after different imbibition times. Peruvian Journal of Agronomy, 1(1), 32-38. https://www.google.com/url?sa=t&rct=j&q=&esrc=s&source=web&cd=&cad=rja&uact=8&ved=2ahUKEwjoiJexjvr0AhW1VTABHVOGC_oQFnoECAUQAQ&url=http%3A%2F%2Frevistas.lamolina.edu.pe%2Findex.php%2Fjpagronomy%2Farticle%2Fdownload%2F1065%2Fpdf_4&usg=AOvVaw2rteIxueKMsqypaLdtZcBx
Martínez, E., & Reyes, O. (2015). Caracterización de la vegetación de la meseta de San Felipe en Camagüey, Cuba, con propósitos de conservación. Revista del Jardín Botánico Nacional, 36, 19-30. http://www.rjbn.uh.cu/index.php/RJBN/article/view/185/0
Méndez, D. 2019. Actividad antioxidante y bacteriostática de Coccoloba cowellii Britton. (Tesis presentada en opción al Título Académico de Máster en Biotecnología. Universidad de Oriente). Santiago de Cuba. Cuba.
Méndez, D., Molina Pérez, E., Spengler, I., Escalona-Arranz, J., & Cos, P. (2019). Chemical composition and antioxidant activity of Coccoloba cowellii Britton. Revista Cubana de Química, 31(2), 185-198. http://scielo.sld.cu/scielo.php?script=sci_abstract&pid=S2224-4212019000200185&lng=en&nrm=iso&tlng=en
Méndez, I., Castillo, M., Trujillo, R., & Martínez, V. (1988). Algunas consideraciones acerca de las plantas serpentinícolas presentes en la flora de Sierra de Cubitas. Revista del Jardín Botánico Nacional, Universidad de La Habana, 9(2), 65-70. https://www.jstor.org/stable/42596854
Méndez, I., Risco, R., & Reyes, M. (2005). Flora and vegetation of the ultramafic outcrop at Camagüey, Cuba. En R. Boyd, A. Baker and J. Proctor, (Eds.), Memorias de la IV Conferencia Internacional sobre Ecología de Serpentina: 21-26 abril 2003, en Science Reviews, Reino Unido, 91 – 96.
Méndez, J. (2016). Osmoacondicionamiento de semillas de Beaucarnea gracilis Lem. como tratamiento pregerminativo. (Tesis presentada en opción al Título Académico de Máster en Biotecnología. Universidad Autónoma Metropolitana Iztapalapa). México D.F. México. https://repository.unad.edu.co/bitstream/handle/10596/11217/1114453102.pdf?sequence=1&isAllowed=y
Naciones Unidas. (2018). La Agenda 2030 y los Objetivos de Desarrollo Sostenible: una oportunidad para América Latina y el Caribe. LC/G.2681-P/Rev.3. Santiago. https://www.cepal.org/es/temas/agenda-2030-desarrollo-sostenible
Sánchez, J., Pernús, M., Torres-Arias, Y., Barrios, D., & Dupuig, Y. (2019). Dormancia y germinación en semillas de árboles y arbustos de Cuba: implicaciones para la restauración ecológica. Acta Botánica Cubana, 218 (2), 77-118. http://revistasgeotech.com/index.php/abc/article/view/290
Soledad, M. (2018). Calidad morfo-fisiológica y sanitaria de semillas de Ruprechtia apetala WEDD. (Polygonaceae) para su domesticación. (Tesis Para optar al Grado Académico de Magister en Ciencias Agropecuarias. Universidad Nacional de Córdoba). Córdova, Argentina. https://rdu.unc.edu.ar/handle/11086/6505
Suárez, D., & Melgarejo, L. (2010). Biología y germinación de semillas. Laboratorio de fisiología y bioquímica vegetal. Departamento de biología. Universidad Nacional de Colombia. https://www.researchgate.net/publication/258627099
Vargas-Simón, G., & Pire, R. (2010). Efecto de dos condiciones de almacenamiento en la germinación de semillas de uva de playa (Coccoloba uvifera (L.) Jacq.). Revista. Facultad Agronomía (LUZ). 27, 559-573. https://www.researchgate.net/publication/265597554_Efecto_de_dos_condiciones_de_almacenamiento_en_la_germinacion_de_semillas_de_uva_de_playa_Coccoloba_uvifera_L_Jacq
Wotton, D. (2018). Seed germination, dormancy and longevity in the endangered shrub Muehlenbeckia astonii (Polygonaceae). New Zealand Journal of Botany, 56 (3), 331-341. http://dx.doi.org/10.1080/0028825X.2018.1491862