Anthelminthic Effect of Dichrostachys cinerea on Infectious L3 Larvae of Cyathostomes Resistant to Albendazol.

  • Lester A. Aguilera-Valle Faculty of Agricultural Sciences, University of Camagüey, Cuba.
  • Anay Delgado-Martínez Faculty of Agricultural Sciences, University of Camagüey, Cuba.
  • Josmel Salas-Romero Faculty of Agricultural Sciences, University of Camagüey, Cuba.
Palabras clave: parasitic nematodes, Cyasthostomes, hatching, third-stage larvae


In vitro  anthelminthic  effect of raw aqueous extracts from leaves and bark of  Dichrostachys cinerea on infectious L3  larvae of  Cyathostomes resistant to Albendazol was evaluated. An inhibition assay for larval hatching was made. Third-stage larvae (L3) were exposed to raw aqueous extracts from leaves (4 mg/ml), and bark (6.8 mg/ml) of Dichrostachys cinerea, for three hours, then hatching was induced. Observations were made every 10 min. Paired T-Student was used to determine the differences in per cent of hatched larvae between the control and the treatment groups.  GraphPadPrism 5.0.0 was used in the study. Significant inhibition levels were observed in all the extracts (P < 0.05)  during the process; whereas hatching in the control group was 100 %, for  the leaf and bark extracts of D. cinerea were 24.05, and 12.56 %, respectively. The results suggest the use of D. cinerea as an alternative anthelminthic treatment to Albendazol-resistant Cyasthostome populations.


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