Effects of Two GnRH-based Recombinant Vaccine Candidates on Pig Fertility

  • Roberto Basulto Baker Center of Genetic Engineering and Biotechnology (CIGB), Camagüey, Cuba
  • Gerardo Guillén Nieto Center of Genetic Engineering and Biotechnology (CIGB), Havana, Cuba
  • Lesvia Calzada Aguilera Center of Genetic Engineering and Biotechnology (CIGB), Camagüey, Cuba
  • Jesús Junco Barranco Center of Genetic Engineering and Biotechnology (CIGB), Camagüey, Cuba
  • Nelson Izquierdo Pérez Faulty of Agricultural Sciences Ignacio Agramonte Loynaz University of, Camagüey, Cuba
  • Osvaldo Reyes Acosta Center of Genetic Engineering and Biotechnology (CIGB), Havana, Cuba
  • Hilda Garay Pérez Center of Genetic Engineering and Biotechnology (CIGB), Havana, Cuba
  • Ricardo Bringas Pérez Center of Genetic Engineering and Biotechnology (CIGB), Havana, Cuba
  • Eulogio Pimentel Vázquez Center of Genetic Engineering and Biotechnology (CIGB), Camagüey, Cuba
  • José A. Bertot Faulty of Agricultural Sciences Ignacio Agramonte Loynaz University of, Camagüey, Cuba
  • Rafael Avilés Faulty of Agricultural Sciences Ignacio Agramonte Loynaz University of, Camagüey, Cuba
  • María Dolores Castro Santana Center of Genetic Engineering and Biotechnology (CIGB), Camagüey, Cuba
  • Eddy Bover Fuentes Center of Genetic Engineering and Biotechnology (CIGB), Camagüey, Cuba
  • Franklin Fuentes Aguilar Center of Genetic Engineering and Biotechnology (CIGB), Camagüey, Cuba

Resumen

This paper describes for the first time the generation of the K88ab-GnRH hybrid fimbria, the fusion of N. meningitidis P64k protein (P64k-GnRH), and its evaluation as vaccine candidates to control fertility in mammals. Twenty hybrid male pigs were randomly distributed in four groups: placebos and immunized with K88ab-GnRH, P64k-GnRH and a GnRH analogue (GnRHm1), linked to a tetanus toxoid (TT) T-helper epitope (positive control), respectively. The pigs were immunized at 9-10 weeks of age, using a two-dose scheme, and were sacrificed sixteen weeks later. K88ab-GnRH, P64k-GnRH, and GnRHm1-TT induced higher, similar, and lower testosterone levels in the serum, compared to the placebo, respectively. In the K88ab-GnRH group, the pigs underwent a reduction in testicle size and weight (P < 0.01), and a reduction in the weight of epididymes compared to the placebo; none of them was able to ejaculate. In the P64k-GnRH group, the pigs had a reduction in testicle weight (P < 0.05), and only one of them was able to ejaculate. The testicles of the pigs immunized with K88ab-GnRH and P64k-GnRH showed structural and functional damage; spermatogenesis was also affected. The accessory sexual glands of the P64k-GnRH group were normal, in contrast to K88ab-GnRH, where interstitial fibrosis was observed. The damage caused by K88ab-GnRH and P64k-GnRH in the target organs evaluated were in all cases lower than the affectations caused by the GnRHm1-TT peptide.

Publicado
2018-09-27
Cómo citar
Basulto Baker, R., Guillén Nieto, G., Calzada Aguilera, L., Junco Barranco, J., Izquierdo Pérez, N., Reyes Acosta, O., Garay Pérez, H., Bringas Pérez, R., Pimentel Vázquez, E., Bertot, J. A., Avilés, R., Castro Santana, M. D., Bover Fuentes, E., & Fuentes Aguilar, F. (2018). Effects of Two GnRH-based Recombinant Vaccine Candidates on Pig Fertility. Revista De Producción Animal, 30(3), 49-57. Recuperado a partir de https://revistas.reduc.edu.cu/index.php/rpa/article/view/2507
Sección
Genética y Reproducción