Effects of Two GnRH-based Recombinant Vaccine Candidates on Pig Fertility

  • Roberto Basulto Baker Center of Genetic Engineering and Biotechnology (CIGB), Camagüey, Cuba
  • Gerardo Guillén Nieto Center of Genetic Engineering and Biotechnology (CIGB), Havana, Cuba
  • Lesvia Calzada Aguilera Center of Genetic Engineering and Biotechnology (CIGB), Camagüey, Cuba
  • Jesús Junco Barranco Center of Genetic Engineering and Biotechnology (CIGB), Camagüey, Cuba
  • Nelson Izquierdo Pérez Faulty of Agricultural Sciences Ignacio Agramonte Loynaz University of, Camagüey, Cuba
  • Osvaldo Reyes Acosta Center of Genetic Engineering and Biotechnology (CIGB), Havana, Cuba
  • Hilda Garay Pérez Center of Genetic Engineering and Biotechnology (CIGB), Havana, Cuba
  • Ricardo Bringas Pérez Center of Genetic Engineering and Biotechnology (CIGB), Havana, Cuba
  • Eulogio Pimentel Vázquez Center of Genetic Engineering and Biotechnology (CIGB), Camagüey, Cuba
  • José A. Bertot Faulty of Agricultural Sciences Ignacio Agramonte Loynaz University of, Camagüey, Cuba
  • Rafael Avilés Faulty of Agricultural Sciences Ignacio Agramonte Loynaz University of, Camagüey, Cuba
  • María Dolores Castro Santana Center of Genetic Engineering and Biotechnology (CIGB), Camagüey, Cuba
  • Eddy Bover Fuentes Center of Genetic Engineering and Biotechnology (CIGB), Camagüey, Cuba
  • Franklin Fuentes Aguilar Center of Genetic Engineering and Biotechnology (CIGB), Camagüey, Cuba

Resumen

This paper describes for the first time the generation of the K88ab-GnRH hybrid fimbria, the fusion of N. meningitidis P64k protein (P64k-GnRH), and its evaluation as vaccine candidates to control fertility in mammals. Twenty hybrid male pigs were randomly distributed in four groups: placebos and immunized with K88ab-GnRH, P64k-GnRH and a GnRH analogue (GnRHm1), linked to a tetanus toxoid (TT) T-helper epitope (positive control), respectively. The pigs were immunized at 9-10 weeks of age, using a two-dose scheme, and were sacrificed sixteen weeks later. K88ab-GnRH, P64k-GnRH, and GnRHm1-TT induced higher, similar, and lower testosterone levels in the serum, compared to the placebo, respectively. In the K88ab-GnRH group, the pigs underwent a reduction in testicle size and weight (P < 0.01), and a reduction in the weight of epididymes compared to the placebo; none of them was able to ejaculate. In the P64k-GnRH group, the pigs had a reduction in testicle weight (P < 0.05), and only one of them was able to ejaculate. The testicles of the pigs immunized with K88ab-GnRH and P64k-GnRH showed structural and functional damage; spermatogenesis was also affected. The accessory sexual glands of the P64k-GnRH group were normal, in contrast to K88ab-GnRH, where interstitial fibrosis was observed. The damage caused by K88ab-GnRH and P64k-GnRH in the target organs evaluated were in all cases lower than the affectations caused by the GnRHm1-TT peptide.

Publicado
2018-09-27
Sección
Genética y Reproducción