Phenotypic characterization of autochthonous strains of enterotoxigenic Escherichia coli 987P
Abstract
The use of a gene fragment that codifies for 987P fimbrial subunit as a DNA probe for 987P+ native strains detection from 117 E. coli isolates, in swine farms in Camagüey, is herein reported. Out of 77 neonates tested, 4 (5,2 %) proved to be infected with E. coli strains carrying the 987P fimbrial gene by ELISA and Western-Blot. Keeping in mind the obtained results we propose to use the DNA-DNA hybridization in order to carry out a more extensive epizootiological study, with the objective of knowing the degree of incidence of the ETEC 987P on porcine diarrhea in Cuba.
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References
BASULTO, R.; L. CALZADA, T. OLIVERA Y J. DE LA FUENTE: Detection of Genes for Fimbrial Antigens in Escherichia coli Isolated from Piglets with Diarrhea in Camagüey. Revista Biotecnología Aplicada. 14 (4): 237-241, 1997. BURNETTE, W. N.: "Western Blotting": Electroforetic Transfer of Proteins from SDS-PAGE to Unmodified Nitrocellulose and Radiographic Detection with Antibody and Radioiodinated Protein A. Anal. Biochem. 112: 195-203, 1981. DE GRAAF, F. K. Y P. KLAASEN: Nucleotide Sequence of the Gen Encoding 987P Fimbrial Subunit of E. coli. FEMS Microbiol. Lett. 42: 253-258, 1987. DEAN, E. A.: Comparison of Receptors for 987P Pili of Enterotoxigenic Escherichia coli in the Small Intestines of Neonatal and Older Pigs. Infect. Immunol. 58: 4030-4035, 1990. FEINBERG, A. P. Y B. A. VOGELSTEIN: A Technique for Radiolabelling DNA Restriction Endonuclease Fragment to High Activity. Anal. Biochem. 132: 6-13, 1984. GUINÉE, P. A. M.; W. H. JANSEN Y C. M. AGTERBERG: Detection of the K99 Antigen by Means of Agglutination and Immunoelectrophoresis in E. coli IsolateD from Calves and its Correlation with Enterotoxigenicity. Infect. Immun. 13: 13691377, 1976. HAREL, J.; H. LAPOINTE, A. FALLARA, L. A. LORTIE, M. BIGRAS-POULIN, S. LARIVIERE Y
J. M. FAIRBROTHER: Detection of Genes for Fimbrial Antigens and Enterotoxins Associated with E. coli Serogroup Isolated from Pigs with Diarrhea. J. Clin. Microbiol. 29: 745-752, 1991. MOL, O. Y B. OUDEGA: Molecular and Structural Aspects of Fimbriae Biosynthesis and Assembly in Escherichia coli. FEMS Microbiol. Rev. 19: 25-52, 1996. MOURICOUT, M.: Swine and Cattle Enterotoxigenic Escherichia coli Mediated Diarrhea. Development of Therapies Based on Inhibition of Bacteria-Host Interactions. Eur. J. Epidemiol. 7 (6): 588-604, 1991. RAYBOULD, T. J. G.; C. F. CROUCH Y S. D. ACRES: Monoclonal Antibody Passive Hemaglutination and Capture ELISA for Direct Detection and Quantitation of F41 and K99 Fimbrial Antigens in ETEC. J. Clin. Microbiol. 25: 278-284, 1987. SAMBROOK, J.; E. F. FRITSCH Y T. MANIATIS: Molecular Cloning: a Laboratory Manual, 3, 2 nd ed., p. 18.47-18.45. Cold Spring Harbor Laboratory Press. USA, 1989. SODERLIND, O.; B. THAFYELIN Y R. MOLLBY: Virulence Factor in E. coli Strain Isolated from Swedish Piglets with Diarrhea. J. Clin. Microbiol. 26: 879-884, 1988. VAN DER WOUDE, M.W.; F. K. DE GRAAF Y H. W. VERSEVELD: Production of the Fimbrial Adhesin 987P by ETEC During Growth under Controlled Conditions in a Chemostat. J. Gen. Microbiol. 135: 3421-3429, 1989.
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