Immunodominance of epitope “a” of fimbriae K88
Abstract
Escherichia coli strain showing K88 fimbria provoke the highest number of coli infections in newborn piglets around the world. Three serologically different K88 fimbria have been identified: K88ab, K88ac and K88 ad. Letter “a” refers to common epitopes carried by every serotype, while “b”, “c”, and “d” refer to epitopes having a specific serotype. Several monoclonal antibodies mostly joining to conformational epitopes present in region “a” from K88 fimbria were obtained. No matter the different conditions used for immunization, “a” continued being immunodomi- nant. Antibodies obtained from this region showed two different epitopes at least. The monoclonal antibodies could be used designing diagnosis systems.
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BAKKER, D.; F. G. VAN ZIJDERVELD, S. VAN DER VEEN, B. OUDEGA Y F. K. DE GRAAF: K88 Fimbriae as Carriers of Heterologous Anti- genic Determinants, Microb. Pathog., May, 8 (5):
-52, 1990.
BAKKER D.; P.T. WILLEMSEN, L. H. SIMONS, F.G. VAN ZIJDERVELD Y F. K. DE GRAAF: Characterization of the antigenic and adhesive properties of FaeG, the major subunit of K88 Fim- briae, Mol. Microbiol., Jan; 6 (2): 247–55, 1992.
BAKKER, D. R.; L. O. BILLEY Y D. H. FRANCIS: Distribution of K88 Escherichia coli-Adhesive and Nonadhesive Phenotypes Among Pigs of Four Breeds, Vet. Microbiol., 54: 123-132, 1997.
CAMPAL, A. C.; M. D. CASTRO, J. A. JUNCO Y S.
CASAS: Caracterización parcial del epítope de la fimbria K88ab de la Escherichia coli (ECET) reco- nocido por un anticuerpo monoclonal dirigido hacia la adhesina, Revista de Producción Animal, Uni- versidad de Camagüey, Cuba. 15 (2), 2003 (en prensa).
DUARTE C.; M. E. FERNÁNDEZ DE COSSIO, G.
SIERRA, E. PENTÓN, A. AGRAZ, G. FURRAZOLA et al.: Anticuerpos monoclonales de ratón contra el interferón recombinante alfa-2. Su
empleo en la purificación y detección del antígeno,
Interferón y Biotecnología, 4: 221-232, 1987. DYKES, C. W.; I. J. HALLIDAY, M. J. READ, A. N.
HOBDEN Y S. HARFORD: Nucleotide Sequences of Four Variants of the K88 Gene of Porcine En-
terotoxigenic Escherichia coli, Infect. Immun., 50:
-283, 1985.
FOGED N. T.; P. KLEMM, F. ELLING, S. E.
JORSAL Y J. ZEUTHEN: Monoclonal Antibodies to K88ab, K88ac and K88ad Fimbriae from Entero- toxigenic Escherichia coli, Microb. Pathog., Feb, 1
(1): 57–69, 1986.
FRANCIS, D. H.: Post-Weaning Escherichia coli Syn- drome: Laboratory Perspectives, p. 18, Proc Swine Dis Conf Swine Pract. Hassan, EAD, LJ Braun, CCL Chase, 2002.
DE GRAAF, F. K. E I. ROORDA: Production, Purifi- cation and Characterization of the Fimbrial Adhe- sive Antigen F41 Isolated from Calf Enteropatho- genic Escherichia coli Strain B41M, Infect. Im- mun., 36: 751, 1982.
GAASTRA, W., Y P. AMSTRU-PEDERSEN: Se- rologic Variants of the K88 Antigen, In D. L. Lark, and S. Normark (ed.), Protein-Carbohydrate Inter- actions in Biological Systems, pp. 95-102, Aca- demic Press Inc., London, England, 1987.
GONZÁLEZ, E. A.; F. VÁZQUEZ, J. IGNACIO GARABAL Y J. BLANCO: Isolation of K88 Anti- gen Variants (ab, ac, ad) from Porcine Enterotoxi- genic Escherichia coli Belonging to Different Sero- types, Microbiol. Immunol., 39: 937-942, 1995.
GUINÉE, P. A. M. Y W. H. CANSEN: Behavior of Escherichia coli K Antigens K88ab, K88ac, and K88ad in Immunoelectrophoresis, Double Diffu-
sion, and Hemagglutination, Infect. Immun.,
:700-705, 1979
KÖLLER, G. Y C. MILSTEIN: Continuous Culture of Fused Cells Secreting Antibodies of Predefined Specificity, Nature, 256: 495-97, 1975.
LAEMLI, U. K.: Cleavaje of Structural Proteins Dur- ing the Assembly of the Head of Bacteriophage T4, Nature, 277: 680-685, 1970.
LI, Y. Y X. F. LIU: Monoclonal Antibodies Specific for K88ab, K88ac and K88ad Antigens of Es- cherichia coli, Wei Sheng Wu Xue Bao, Oct. 29 (5):
-53, 1989.
LOWRY, O. H.; N. J. ROSEBROUGH, A. L. FARR Y R. J. RANDALL: Protein Measurement with the Folin-Phenol Reagents, J. Biol. Chem., 193: 265-
, 1951.
MOOI, F. R. Y F. K. DE GRAAF: Molecular Biology of Fimbriae of Enterotoxigenic Escherichia coli. Curr Top. Microbiol. Immunol., 118: 119-138,
THIRY, G.; A. CLIPPE, T. SCARCEZ Y J.
PETRE: Cloning of DNA Sequences Encoding
Foreign Peptides and their Expression in the K88
Pili, Appl. Environ, Microbiol., 55: 984-993,1987. THORNS C. J.; C. D. BOARER Y J. A. MORRIS:
Production and Evaluation of Monoclonal Antibod- ies Directed Against the K88 Fimbrial Adhesin Produced by Escherichia coli Enterotoxigenic for
Piglets, Res. Vet. Sci., Sep; 43 (2): 233-8, 1987.
VAN DER BROECK, W.; E. COX Y B. M GODDERIS: Receptor–specific Binding of Puri- fied F4 to Isolate Villi, Vet. Microbiol., Aug 31; 68 (3–4): 255–63, 1999.
VAN DER BROECK, W.; E. COX, B. OUDEGA Y B.
M. GODDEERIS: The F4 Fimbrial Antigen of Es- cherichia coli and its Receptors, Microbiol, Feb.,
(3-4): 223–44, 2000.
WESTERMAN, R. B.; K. W. MILLS, R. M.
PHILLIPS, G. W. FORTNER, Y J. M. GREENWOOD: Predominance of the ac Variant in K88-Positive Escherichia coli Isolates from Swine, J. Clin. Microbiol., 26: 149-150, 1988.
WILSON M. B. Y P. K. NAKANE: The Covalent Coupling of Proteins to Periodate-Oxidized Sephadex: a New Approach to Immunoadsorbent
Preparation, J. Immunol Methods, 12 (1-2): 171-81,
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